Schematic for cDNA synthesis by templateswitching. (Step 1) Primer
Template Switch Oligo. The tso adds a common 5' sequence to. Web tso全称template switch oligo 模板转换寡核苷酸，是一种寡核苷酸，在反转录的过程中由逆转录酶加在非模板链5端的c寡核苷酸，用于下游的cdna扩增。 在第一链合成过程中，当到达 rna 模板的 5' 末端时，mmlv 逆转录酶的末端转移酶活性会在新合成的 cdna 链的 3' 末端.
Schematic for cDNA synthesis by templateswitching. (Step 1) Primer
This primer, in conjunction with a template switching oligo (tso), generates cdnas containing adaptor sequences at both the 5′ and 3′ ends. However, use of a template switching chimeric dna:rna oligo and mmlv reverse transcriptase can improve 5. In the first step, cdna is synthesized using an rt primer that contains an adaptor of known sequence at the 5′ end. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. Reverse transcriptase then switches templates and continues replicating to the end of the oligo. Web the tso or (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. Web use of template switching oligos (ts oligos, tsos) for efficient cdna library construction. Web the cdna synthesis and amplification protocol contains two steps. The tso adds a common 5' sequence to full length cdna that is used for downstream cdna amplification. Web tso全称template switch oligo 模板转换寡核苷酸，是一种寡核苷酸，在反转录的过程中由逆转录酶加在非模板链5端的c寡核苷酸，用于下游的cdna扩增。 在第一链合成过程中，当到达 rna 模板的 5' 末端时，mmlv 逆转录酶的末端转移酶活性会在新合成的 cdna 链的 3' 末端.
The tso adds a common 5' sequence to. Reverse transcriptase then switches templates and continues replicating to the end of the oligo. Web the tso or (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. This primer, in conjunction with a template switching oligo (tso), generates cdnas containing adaptor sequences at both the 5′ and 3′ ends. The tso adds a common 5' sequence to. However, use of a template switching chimeric dna:rna oligo and mmlv reverse transcriptase can improve 5. Web tso全称template switch oligo 模板转换寡核苷酸，是一种寡核苷酸，在反转录的过程中由逆转录酶加在非模板链5端的c寡核苷酸，用于下游的cdna扩增。 在第一链合成过程中，当到达 rna 模板的 5' 末端时，mmlv 逆转录酶的末端转移酶活性会在新合成的 cdna 链的 3' 末端. The tso adds a common 5' sequence to full length cdna that is used for downstream cdna amplification. Web use of template switching oligos (ts oligos, tsos) for efficient cdna library construction. In the first step, cdna is synthesized using an rt primer that contains an adaptor of known sequence at the 5′ end. Web the cdna synthesis and amplification protocol contains two steps.
